Located
on the second floor of the Seeley Mudd Building (Rm 228), this facility
opened in February of 2001 and is dedicated to the sequence analysis
of peptides and proteins by mass spectrometry. Electrospray ionization
is our method of choice for precise mass measurement of biological
molecules. When peptides are analyzed by ion-trap mass spectrometry,
the amino acid sequence is determined by tandem mass spectrometry
(MS/MS) and database searching.
The facility is charged with serving first those investigators
at Harvard Medical School (quad-based), then surrounding affiliated
hospitals and institutes, and finally the Boston scientific community.
We are currently accepting commassie-stained gel bands for sequencing exclusively by microcapillary LC-MS/MS techniques. We are a high-throughput facility utilizing three dedicated LCQ DECA ion-trap mass spectrometers (ThermoFinnigan) with sample turnaround times of typically less than one week. All reports are e-mailed to the investigator as HTML and contain links to the original (raw) data and the databases searched.
Contact
 |
Steven Gygi, Ph.D.E-mail: steven_gygi@hms.harvard.edu |
Page updated: February 14, 2001