Imaging Specialist

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The Kirchhausen lab is seeking an energetic and highly motivated individual for an opening as Imaging Specialist to start immediately. The ideal candidate would be at the B.Sc./Master's or Engineering level, with imaging experience in wide field, TIRF and/or confocal microscopy and expertise in live and/or single molecule visualization. Proven ability to maintain microscopes and build associated instruments are essential. She/he is an individual interested in working with students, post-doctoral fellows and faculty doing research that requires generation of 'high end', state-of-the-art light microscopy imaging data.

Our research program integrates results from molecular biology, structural biology, classical and contemporary cell biology, single-molecule biophysics, live-cell imaging, and ‘chemical genetics’ to describe complex cellular processes at remarkably high spatial and temporal resolution and achieve single-molecule biochemistry in living cells.

The goal is to enable researchers primarily working on cell biology to make use of advanced imaging technologies in their research programs. She/he oversees the day-to-day operations and is responsible for maintaining and directing the operation of six microscopes and associated equipment (for spinning disk confocal microscopy, TIRF, FRAP, expansion imaging, super resolution, single-molecule imaging, photoactivation, deconvolution, computers), for training users and for providing hands-on help and assistance on an ongoing basis. Ability to communicate effectively with researchers with various knowledge levels in imaging technology and proven ability to troubleshoot are essential.

We have two inverted Zeiss microscopes and one upright Zeiss microscope that are coupled to spinning disc confocal heads set up for live cell 3D-time series imaging. One inverted microscope is also set up for TIRF, FRAP and photoactivation. Three additional microscopes are set up for multi-wavelength TIRF of live cells with singlemolecule sensitivity. All the set ups include an environmental chamber with temperature, humidity and CO2 control. Deconvolution and image restoration is done with our own computational cluster. A RAID server is available for data storage.

To apply, please contact:

Tom Kirchhausen
Professor in Cell Biology and Pediatrics
Springer Family Chair
Harvard Medical School/Boston Children’s Hospital
W. Alpert Building Room 133
200 Longwood Ave Boston, MA 02115 USA
e-mail: kirchhausen@crystal.harvard.edu