FIB-SEM Imaging Specialist

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The Kirchhausen Lab is seeking an energetic and highly motivated individual for an opening as Focused Ion Beam – Scanning Electron Microscope (FIB-SEM) Imaging Specialist to start immediately. The ideal candidate would be have a B.Sc. or Master's degree in Bioengineering, Biophysics, Chemistry or Cell Biology, with imaging experience in transmission, scanning or FIB-SEM electron microscopy. Proven ability to independently operate an electron microscope is essential. She/he is an individual interested in working with students, post-doctoral fellows and faculty doing research that requires generation of 'high end', state-of-the-art FIB-SEM imaging data. 

The Kirchhausen Lab's research program integrates results from molecular and structural biology, contemporary cell biology, single-molecule biophysics, live-cell imaging, and ‘chemical genetics’ to describe complex cellular processes at remarkably high spatial and temporal resolution and achieve single-molecule biochemistry in living cells. The ultimate aim is to link molecular structure and mechanism with the physiology of cells and tissues.

The goal is to enable researchers primarily working on cell biology to make use of advanced imaging technologies in their research programs. She/he oversees the day-to-day operations and is responsible for directing, maintaining and operating in a daily basis the FIB-SEM, for guiding in the preparation of samples and image analysis, for training users and for providing hands-on help and assistance on an ongoing basis. Ability to communicate effectively with researchers with various knowledge levels in imaging technology and proven ability to troubleshoot are essential.

The Kirchhausen Lab has a Zeiss Crossbeam 540 FIB-SEM system for EM imaging, a Quorum 150R ES sputter coater for non-oxidizing metal and carbon coating, an automated Leica tissue processing instrument for staining samples. 3D Image restoration and analysis is done off-line with our own computational cluster. A RAID server is available for data storage.

To apply, please contact:

Tom Kirchhausen
Professor in Cell Biology and Pediatrics
Springer Family Chair
Harvard Medical School/Boston Children’s Hospital
W. Alpert Building Room 133
200 Longwood Ave Boston, MA 02115 USA