Opportunities

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Open Positions

Laboratory Research Assistant

The Kirchhausen lab is seeking an energetic and highly motivated individual for an opening as a Laboratory Research Assistant to start immediately. The ideal candidate would be at the B.Sc./Master's level in biological sciences, biochemistry, chemistry, or related discipline.

Our research program integrates results from molecular biology, structural biology, classical and contemporary cell biology, single-molecule biophysics, live-cell imaging, and ‘chemical genetics’ to describe complex cellular processes at remarkably high spatial and temporal resolution and achieve single-molecule biochemistry in living cells. The ultimate goal is to link molecular structure and mechanism with the physiology of cells and tissues.

She/he is an individual interested in doing research that requires use of purified proteins. The goal is to enable researchers primarily working on in vitro reconstitution assays to obtain pure proteins that can then be studied with advanced imaging technologies suited for single-molecule biochemistry in their research programs.

This position requires 1-2 years of experience in performing analytical biochemical assays and protein purification. Demonstrable working knowledge of gel filtration and ionic exchange chromatography, FPLC, spectrophotometry, SDS-PAGE and Western blot analysis is essential. Important for a successful appointment is a collaborative attitude with excellent written and oral communications skills, accurate record keeping and ability to follow instructions. Ability to work and communicate effectively with our team members composed of students and post-doctoral fellows will be essential. Preference will be given to individuals with an analytical mind, attention to detail, and organization skills.

We have 3 state of the art FPLCs, one MALS/HPLC, a Nano-drop spectrophotometer, plate readers, high speed centrifuges and tissue culture equipment for generation of recombinant protein in bacteria and insect cells.

To apply, please contact:

Tom Kirchhausen
Professor in Cell Biology and Pediatrics
Springer Family Chair
Harvard Medical School/Boston Children’s Hospital
W. Alpert Building Room 133
200 Longwood Ave Boston, MA 02115 USA
e-mail: kirchhausen@crystal.harvard.edu

FIB-SEM Imaging Specialist

The Kirchhausen lab is seeking an energetic and highly motivated individual for an opening as Focused Ion Beam – Scanning Electron Microscope (FIB-SEM) Imaging Specialist to start immediately. The ideal candidate would be at the B.Sc./Master's or Engineering level, with imaging experience in transmission, scanning or FIB-SEM electron microscopy. Proven ability to independently operate an electron microscope is essential. She/he is an individual interested in working with students, post-doctoral fellows and faculty doing research that requires generation of 'high end', state-of-the-art FIB-SEM imaging data.

Our research program integrates results from molecular biology, structural biology, classical and contemporary cell biology, single-molecule biophysics, live-cell imaging, and ‘chemical genetics’ to describe complex cellular processes at remarkably high spatial and temporal resolution and achieve single-molecule biochemistry in living cells. The ultimate aim is to link molecular structure and mechanism with the physiology of cells and tissues.

The goal is to enable researchers primarily working on cell biology to make use of advanced imaging technologies in their research programs. She/he oversees the day-to-day operations and is responsible for directing, maintaining and operating in a daily basis the FIB-SEM, for guiding in the preparation of samples and image analysis, for training users and for providing hands-on help and assistance on an ongoing basis. Ability to communicate effectively with researchers with various knowledge levels in imaging technology and proven ability to troubleshoot are essential.

We have a Zeiss Crossbeam 540 FIB-SEM system for EM imaging, a Quorum 150R ES sputter coater for non-oxidizing metal and carbon coating, an automated Leica tissue processing instrument for staining samples. 3D Image restoration and analysis is done off-line with our own computational cluster. A RAID server is available for data storage.

To apply, please contact:

Tom Kirchhausen
Professor in Cell Biology and Pediatrics
Springer Family Chair
Harvard Medical School/Boston Children’s Hospital
W. Alpert Building Room 133
200 Longwood Ave Boston, MA 02115 USA
e-mail: kirchhausen@crystal.harvard.edu

Imaging Specialist

The Kirchhausen lab is seeking an energetic and highly motivated individual for an opening as Imaging Specialist to start immediately. The ideal candidate would be at the B.Sc./Master's or Engineering level, with imaging experience in wide field, TIRF and/or confocal microscopy and expertise in live and/or single molecule visualization. Proven ability to maintain microscopes and build associated instruments are essential. She/he is an individual interested in working with students, post-doctoral fellows and faculty doing research that requires generation of 'high end', state-of-the-art light microscopy imaging data.

Our research program integrates results from molecular biology, structural biology, classical and contemporary cell biology, single-molecule biophysics, live-cell imaging, and ‘chemical genetics’ to describe complex cellular processes at remarkably high spatial and temporal resolution and achieve single-molecule biochemistry in living cells.

The goal is to enable researchers primarily working on cell biology to make use of advanced imaging technologies in their research programs. She/he oversees the day-to-day operations and is responsible for maintaining and directing the operation of six microscopes and associated equipment (for spinning disk confocal microscopy, TIRF, FRAP, expansion imaging, super resolution, single-molecule imaging, photoactivation, deconvolution, computers), for training users and for providing hands-on help and assistance on an ongoing basis. Ability to communicate effectively with researchers with various knowledge levels in imaging technology and proven ability to troubleshoot are essential.

We have two inverted Zeiss microscopes and one upright Zeiss microscope that are coupled to spinning disc confocal heads set up for live cell 3D-time series imaging. One inverted microscope is also set up for TIRF, FRAP and photoactivation. Three additional microscopes are set up for multi-wavelength TIRF of live cells with singlemolecule sensitivity. All the set ups include an environmental chamber with temperature, humidity and CO2 control. Deconvolution and image restoration is done with our own computational cluster. A RAID server is available for data storage.

To apply, please contact:

Tom Kirchhausen
Professor in Cell Biology and Pediatrics
Springer Family Chair
Harvard Medical School/Boston Children’s Hospital
W. Alpert Building Room 133
200 Longwood Ave Boston, MA 02115 USA
e-mail: kirchhausen@crystal.harvard.edu

Research Associate

The Department of Cell Biology at Harvard Medical School is seeking a highly skilled scientist to join the Cellular Dynamics and Neurodegeneration Initiative. The Research Associate will work with a team of scientists who are seeking to build an integrative platform for understanding cell biological processes that are implicated in disease.

The job responsibility will include but not limited to: mammalian cell culture, PCR, molecular cloning, DNA and RNA extraction, DNA sequence analysis, Western blot, flow cytometry, chemical transfection and electroporation of cultured cells.  You will also be expected to analyze and present your data to the manager and colleagues.  

The successful candidate will have a Bachelor‘s degree and above in biology or the biomedical sciences. Extensive experience in mammalian cell culture and molecular biology is essential. Experience with human pluripotent stem cell maintenance and differentiation is preferred. Experience with protein purification or karyotyping is a plus. You must be self-motivated, able to work independently as well as part of a team, have excellent communication and interpersonal skills, pay attention to detail, and be highly organized.

If you are interested in this exciting career opportunity to work with the vibrant community of cell biologists at Harvard Medical School, please email a cover letter and CV to cellbiologyhms@gmail.com. In addition, please have three referees send letters of recommendation directly to cellbiologyhms@gmail.com.

 

Harvard University is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veteran status, or any other characteristic protected by law.

 

Advanced Microscopy & Core Facility Management Post-Doctoral Training Fellowship

The Nikon Imaging Center at Harvard Medical School (NIC@HMS) is seeking candidates for a 2-3 year (renewable annually) post-doctoral training fellowship in advanced optical microscopy techniques and core facility management.

Optical microscopy has become central to progress in many areas of science. At the same time, the complexity of instruments and quantitative imaging experiments has dramatically increased, with many requiring extensive expertise to operate. Microscopy facilities managed by PhD-level scientists who advise and train researchers on imaging experimental design, the best instruments to use for their experiments, and proper use of instruments have become essential sources of expertise in many research institutions, and core facility management has become a stimulating career path for scientists with experience in advanced quantitative microscopy techniques and an interest in facilitating science broadly.

The NIC@HMS Fellow will learn advanced quantitative microscopy techniques including confocal, TIRF, FRET, FRAP, photo-activation, single-molecule imaging, light sheet and super-resolution microscopy, and key skills needed to manage a large, heavily-used core facility. The Fellow will learn to train core facility users to select and apply the appropriate techniques, using a wide range of biological specimens and experimental approaches. The Fellow will also have the opportunity to identify additional responsibilities that match her/his interests, such as: organizing and teaching microscopy courses in the NIC@HMS Educational Program; organizing microscopy discussion groups/journal clubs; troubleshooting equipment problems; developing protocols for testing equipment performance; designing and/or implementing novel or custom imaging techniques.

We are seeking candidates with optical microscopy experience, a PhD in biology, physics, or a related discipline, and interest in a career in optical microscopy. For more information and to apply, please visit microfellows.hms.harvard.edu.